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| Burge, Colleen A.; Friedman, Carolyn S.; Getchell, Rodman; House, Marcia; Lafferty, Kevin D.; Mydlarz, Laura D.; Prager, Katherine C.; Sutherland, Kathryn P.; Renault, Tristan; Kiryu, Ikunari; Vega-thurber, Rebecca. |
| Linking marine epizootics to a specific etiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative realtime PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease... |
| Tipo: Text |
Palavras-chave: Marine disease; Aetiology; Diagnostics; Marine epizootics. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00314/42536/41911.pdf |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A.. |
| Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the... |
| Tipo: Text |
Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf |
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| Burge, Colleen A.; Reecez, Kimberly S.; Dhar, Arun K.; Kirkland, Peter; Morga, Benjamin; Degremont, Lionel; Faury, Nicole; Wippel, Bryanda J. T.; Macintyre, Alanna; Friedman, Carolyn S.. |
| Economically devastating mortality events of farmed and wild shellfish due to infectious disease have been reported globally. Currently, one of the most significant disease threats to Pacific oyster Crassostrea gigas culture is the ostreid herpesvirus 1 (OsHV-1), in particular the emerging OsHV-1 microvariant genotypes. OsHV-1 microvariants (OsHV-1 mu vars) are spreading globally, and concern is high among growers in areas unaffected by OsHV-1. No study to date has compared the relative virulence among variants. We provide the first challenge study comparing survival of naive juvenile Pacific oysters exposed to OsHV-1 mu vars from Australia (AUS mu var) and France (FRA mu var). Oysters challenged with OsHV-1 mu vars had low survival (2.5%a exposed to AUS... |
| Tipo: Text |
Palavras-chave: Ostreid herpesvirus 1; Microvariant; OsHV-1 mu vars; POMS; Pacific oyster; Crassostrea gigas; Viral disease; QPCR; Alkalinity; Emerging infectious diseases. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00631/74277/73889.pdf |
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| Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Dégremont, Lionel; Burge, Colleen A.; Mancilla Cortez, Daniel; Friedman, Carolyn S.; Fleener, Gary B.; Dumbauld, Brett R.; Langdon, Chris. |
| Background Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in... |
| Tipo: Text |
Palavras-chave: Ostreid herpesvirus 1; Crassostrea gigas; Heritability; Breeding. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00598/70974/69206.pdf |
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| Burge, Colleen; Judah, Linda; Conquest, Loveday; Griffin, Frederick; Cheney, Daniel; Suhrbier, Andrew; Vadopalas, Brent; Olin, Paul G.; Renault, Tristan; Friedman, Carolyn S.. |
| Summer seed mortality (SSM) has occurred yearly in Tomales Bay, California since 1993. SSM has resulted in up to 90% cumulative losses, and has been associated with extreme temperature, phytoplankton blooms, and an oyster herpesvirus. In this study, three stocks of Pacific oysters were planted at three sites in California (Inner Tomales Bay, Outer Tomales Bay, and Bodega Harbor) in October of 2000 (Fall) and April of 2001 (Spring) and monitored for mortality, growth, and health status. In April of 2001, a similar study was conducted in Totten Inlet, WA state using cohorts of oysters planted in California; animals were monitored for mortality and growth. Temperature data were collected at all sites; phytoplankton abundance data were collected at the... |
| Tipo: Text |
Palavras-chave: California; Tomales Bay; Oyster herpesvirus; Temperature; Mortality; Crassostrea gigas; Pacific oysters. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2976.pdf |
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